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Image Search Results
Journal: bioRxiv
Article Title: Evolutionary fingerprint in rodent PD1 confers weakened activity and enhanced tumor immunity compared to human PD1
doi: 10.1101/2024.09.21.614250
Figure Lengend Snippet: Left, FACS histograms showing hu PD1 expressions on anti-CD3ε/anti-CD28 stimulated human primary CD4 + T cells and on stable Jurkat cells transduced with hu PD1 driven by either an dSV40 (weak) or an SFFV (strong) promoter. Right, PD1 densities on human primary CD4 + T cells plotted against duration of stimulation by anti-CD3/anti-CD28 beads. Dashed lines indicate the densities of SFFV- and dSV40-driven PD1 expressions on Jurkat cells. PD1 densities were calculated using molecular numbers quantified with a Quantum PE MESF kit and diameters of cells (human primary CD4 + T cell: 4.8 µm, Jurkat cells: 15 µm).
Article Snippet: The absolute amount of PD1 surface expressions on human primary CD4 + T cells or on Jurkat cells was quantified using a
Techniques: Transduction